primary antibodies against hif 1α Search Results


anti-hif-1α antibodies  (WuXi AppTec)
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WuXi AppTec anti-hif-1α antibodies
( a ) Detection of the mRNA levels of <t>HIF-1α,</t> pVHL and VEGF by semi-quantitative RT-PCR. ( b ) Western blot analysis of HIF-1α and pVHL protein expression. The numbers below the lanes indicate the levels of mRNA or protein expression compared with the control levels. ( c ) The level of VEGF secretion into the cell culture supernatants was analysed by ELISA. ( d ) Effect of SAL on the transcriptional activity of HIF-1α. The relative luciferase activity level was measured and normalized to the enzymatic activity level of β-galactosidase. ( e ) Effect of SAL on the translocation of HIF-1α. After culturing in the presence of 100 nM SAL or vehicle followed by treatment with CoCl 2 for 24 h, MG-63 cells were treated as described in the Methods section. Then, the nuclear localization of HIF-1α was observed via confocal microscopy. The position of the cell nucleus was confirmed by staining with DAPI (blue), and HIF-1α expression was detected as FITC staining (green) (Original section, 400×). ** P < 0.01 compared with vehicle control; # P < 0.05, ## P < 0.01 compared with CoCl 2 .
Anti Hif 1α Antibodies, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+hif+1%CE%B1/pmc04997314-19-0-7?v=WuXi+AppTec
Average 90 stars, based on 1 article reviews
anti-hif-1α antibodies - by Bioz Stars, 2026-08
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( a ) Detection of the mRNA levels of HIF-1α, pVHL and VEGF by semi-quantitative RT-PCR. ( b ) Western blot analysis of HIF-1α and pVHL protein expression. The numbers below the lanes indicate the levels of mRNA or protein expression compared with the control levels. ( c ) The level of VEGF secretion into the cell culture supernatants was analysed by ELISA. ( d ) Effect of SAL on the transcriptional activity of HIF-1α. The relative luciferase activity level was measured and normalized to the enzymatic activity level of β-galactosidase. ( e ) Effect of SAL on the translocation of HIF-1α. After culturing in the presence of 100 nM SAL or vehicle followed by treatment with CoCl 2 for 24 h, MG-63 cells were treated as described in the Methods section. Then, the nuclear localization of HIF-1α was observed via confocal microscopy. The position of the cell nucleus was confirmed by staining with DAPI (blue), and HIF-1α expression was detected as FITC staining (green) (Original section, 400×). ** P < 0.01 compared with vehicle control; # P < 0.05, ## P < 0.01 compared with CoCl 2 .

Journal: Scientific Reports

Article Title: Protective effect of salidroside against bone loss via hypoxia-inducible factor-1α pathway-induced angiogenesis

doi: 10.1038/srep32131

Figure Lengend Snippet: ( a ) Detection of the mRNA levels of HIF-1α, pVHL and VEGF by semi-quantitative RT-PCR. ( b ) Western blot analysis of HIF-1α and pVHL protein expression. The numbers below the lanes indicate the levels of mRNA or protein expression compared with the control levels. ( c ) The level of VEGF secretion into the cell culture supernatants was analysed by ELISA. ( d ) Effect of SAL on the transcriptional activity of HIF-1α. The relative luciferase activity level was measured and normalized to the enzymatic activity level of β-galactosidase. ( e ) Effect of SAL on the translocation of HIF-1α. After culturing in the presence of 100 nM SAL or vehicle followed by treatment with CoCl 2 for 24 h, MG-63 cells were treated as described in the Methods section. Then, the nuclear localization of HIF-1α was observed via confocal microscopy. The position of the cell nucleus was confirmed by staining with DAPI (blue), and HIF-1α expression was detected as FITC staining (green) (Original section, 400×). ** P < 0.01 compared with vehicle control; # P < 0.05, ## P < 0.01 compared with CoCl 2 .

Article Snippet: Anti-HIF-1α and anti-pVHL antibodies were purchased from Abgent Biotechnology, Inc. (USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay, Luciferase, Translocation Assay, Confocal Microscopy, Staining

Representative sections stained with Hypoxyprobe-1 or antibodies against HIF-1α, VEGF and CD31 are shown (the blue arrows, 400×). Quantitative measurements of the staining intensity of Hypoxyprobe-1 (a1–a5, a ) and the expression levels of HIF-1α (b1–b5, b ), VEGF (c1–c5, c ) and CD31 (d1–d5, d ) are shown. The data are presented as the means ± SD. ** P < 0.01 compared with the sham group; ## P < 0.01 compared with the OVX group.

Journal: Scientific Reports

Article Title: Protective effect of salidroside against bone loss via hypoxia-inducible factor-1α pathway-induced angiogenesis

doi: 10.1038/srep32131

Figure Lengend Snippet: Representative sections stained with Hypoxyprobe-1 or antibodies against HIF-1α, VEGF and CD31 are shown (the blue arrows, 400×). Quantitative measurements of the staining intensity of Hypoxyprobe-1 (a1–a5, a ) and the expression levels of HIF-1α (b1–b5, b ), VEGF (c1–c5, c ) and CD31 (d1–d5, d ) are shown. The data are presented as the means ± SD. ** P < 0.01 compared with the sham group; ## P < 0.01 compared with the OVX group.

Article Snippet: Anti-HIF-1α and anti-pVHL antibodies were purchased from Abgent Biotechnology, Inc. (USA).

Techniques: Staining, Expressing